5/2/2023 0 Comments Hans kohler converter 2Only one PBCDen stereoisomer was formed by LinA2, while LinA1 and LinATM produced mixtures of two PBCDE enantiomers at three times lower rates than LinA2. Rate constants k cat and enzyme specificities k cat/ K M indicate that ε-HBCD conversion was fastest and most specific with LinA2. Transformations followed Michaelis-Menten kinetics. LC-MS data indicate that all enzymes converted ε-HBCD to pentabromocyclododecenes (PBCDens). ![]() Furthermore, we compared kinetics and selectivity of these LinA variants with respect to ε-HBCD. We studied the reactivity of those eight HBCD stereoisomers found in technical HBCD. These enzymes are involved in the hexachlorocyclohexane (HCH) metabolism, specifically of the insecticide γ-HCH (Lindane). We expressed three LinA dehydrohalogenase enzymes in E. coli bacteria, two wild-type, originating from Sphingobium indicum B90A bacteria and LinATM, a triple mutant of LinA2, with mutations of L96C, F113Y and T133 M. This makes ε-HBCD an interesting substrate to study the selectivity of biotransformations. ![]() Among the 16 stereoisomers known, ε-HBCD has the highest symmetry. ![]() Hexabromocyclododecanes (HBCDs) were used as flame-retardants until their ban in 2013.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |